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3 In addition to disrupting calcium metabolism, lead can mimic and displace magnesium and iron from certain enzymes that construct the building blocks of DNA (nucleotides) and disrupt the activity of zinc in the synthesis of heme (the carrier of oxygen in red blood cells)

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Cells were spun down at 100 rcf for 3 min and each single microwell contained at least 5000 cells, cultured in E8F medium (without antibiotic) containing 10 M ROCK inhibitor Y-27632 (10 mM stock, Stemcell Technologies, USA #72304)